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(1→3),(1→4)‐ß‐d‐Glucans in the cell walls of the Poales (sensu lato): an immunogold labeling study using a monoclonal antibody
Author(s) -
Trethewey Jason A. K.,
Campbell Lisa M.,
Harris Philip J.
Publication year - 2005
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.92.10.1660
Subject(s) - sensu , immunogold labelling , biology , monoclonal antibody , sensu stricto , botany , antibody , zoology , ultrastructure , genetics , genus
(1→3),(1→4)‐ß‐Glucans had previously been detected in nonlignified cell wall preparations of only the Poaceae and five other families in the graminoid clade of the Poales ( s.l .). Cell walls of vegetative organs of 12 species in nine families of the Poales ( s.l .) were examined by immunogold labeling using a monoclonal antibody to (1→3),(1→4)‐ß‐glucans. Three types of wall‐labeling patterns were identified depending on the density of labeling of the nonlignified walls of epidermal and parenchyma cells and the lignified walls of sclerenchyma fibers and xylem tracheary elements: type 1 in Poaceae and Flagellariaceae, type 2 in Restionaceae and Xyridaceae, and type 3 in Cyperaceae and Juncaceae. Type 1 had the heaviest labeling of nonlignified walls and type 2 the heaviest labeling of lignified walls. Type 3 had the least wall labeling, with only very light labeling of nonlignified and lignified walls. No labeling was found over walls of Typhaceae, Sparganiaceae, or Bromeliaceae. The results are discussed in relation to Poales phylogeny.