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The Effect of NaCl on growth, N 2 fixation (acetylene reduction), and percentage total nitrogen in Leucaena leucocephala (Leguminosae) var. K‐8
Author(s) -
Anthraper Annie,
DuBois John D.
Publication year - 2003
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.90.5.683
Subject(s) - leucaena leucocephala , biology , leucaena , nitrogen fixation , nitrogen , agronomy , zoology , botany , chemistry , organic chemistry , genetics , bacteria
Leucaena leucocephala var. K‐8 is a fast‐growing, tropical leguminous tree that has multiple economic uses. This study was conducted to evaluate the effect(s) of varying NaCl concentrations on growth, N 2 fixation, and percentage of total tissue nitrogen in different organs in L. leucocephala . Seeds were germinated and grown for 10 wk with a nitrogen‐free fertilizer applied every 2 wk. At 10 wk, plants were treated for either 0, 7, 14, 21, or 28 wk with either deionized water (control), 0.00625 mol/L, 0.0125 mol/L, 0.025 mol/L, 0.05 mol/L, or 0.1 mol/L NaCl in addition to the fertilizer every 2 wk. Growth was measured as plant height, nodule number and mass, and dry tissue mass. N 2 fixation was measured by the acetylene reduction assay. Percentage of tissue nitrogen was determined using Kjeldahl analysis. In younger plants (7‐wk treatment), major fluctuations in NaCl tolerance were observed in the different plant organs. As plants matured (14‐ and 21‐wk treatment) NaCl concentrations of 0.025 mol/L and higher caused the greatest reduction in growth and tissue nitrogen. We conclude that NaCl concentrations of 0.025 mol/L and greater caused a major decrease in growth, N 2 fixation, and percentage of tissue nitrogen in L. leucocephala plants that were less than 1 yr old.