Origins of domestication and polyploidy in oca ( Oxalis Tuberosa : Oxalidaceae). 2. Chloroplast‐expressed glutamine synthetase data

In continuing study of the origins of the octoploid tuber crop oca, Oxalis tuberosa Molina, we used phylogenetic analysis of DNA sequences of the chloroplast‐active (nuclear encoded) isozyme of glutamine synthetase (ncpGS) from cultivated oca, its allies in the “ Oxalis tuberosa alliance,” and other Andean Oxalis . Multiple ncpGS sequences found within individuals of both the cultigen and a yet unnamed wild tuber‐bearing taxon of Bolivia were separated by molecular cloning, but some cloned sequences appeared to be artifacts of polymerase chain reaction (PCR) recombination and/or Taq error. Nonetheless, three classes of nonrecombinant sequences each joined a different part of the O. tuberosa alliance clade on the ncpGS gene tree. Octoploid oca shares two sequence classes with the Bolivian tuber‐bearing taxon (of unknown ploidy level). Fixed heterozygosity of these two sequence classes in all ocas sampled suggests that they represent homeologous loci and that oca is allopolyploid. A third sequence class, found in eight of nine oca plants sampled, might represent a third homeologous locus, suggesting that oca may be autoallopolyploid, and is shared with another wild tuber‐bearing species, tetraploid O. picchensis of southern Peru. Thus, ncpGS data identify these two taxa as the best candidates as progenitors of cultivated oca.