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The origin of tobacco's T genome is traced to a particular lineage within Nicotiana tomentosiformis (Solanaceae)
Author(s) -
Murad Lena,
Lim K. Yoong,
Christopodulou Vangellis,
Matyasek Roman,
Lichtenstein Conrad P.,
Kovarik Ales,
Leitch Andrew R.
Publication year - 2002
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.89.6.921
Subject(s) - genome , biology , lineage (genetic) , nicotiana tabacum , genetics , evolutionary biology , gene
Nicotiana tabacum (tobacco) is a natural allotetraploid. The maternal genome donor is not controversial and is probably derived from an ancestor of N. sylvestris . The paternal, T‐genome donor has been less clear, with N. tomentosiformis , N. otophora , or an introgression hybrid proposed. Here we provide evidence that the T genome of N. tabacum is derived from a particular lineage of N. tomentosiformis . We show that the repetitive sequences of geminiviral origin, GRD53 and GRD3, are present in the genomes of N. tabacum cultivars, a tobacco cell suspension culture TBY‐2, and N. tomentosiformis ac. NIC 479/84. Surprisingly, they are not present in another three varieties of N. tomentosiformis . A detailed cytogenetic analysis also revealed that N. tomentosiformis ac. NIC 479/84 most closely resembles the N. tabacum T genome in the location of other tandem repetitive sequences. Thus, tobacco formed after divergence within N. tomentosiformis , and the spectrum of potential donors of the paternal genome can be narrowed to a genotype of N. tomentosiformis characterized by the presence of GRD53 and GRD3 repeats. It is clear that future paternity studies in tobacco should use N. tomentosiformis ac. NIC 479/84 rather than any other accession.