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Microsatellite primers for an Amazonian lowland tropical tree, Protium subserratum (Burseraceae)
Author(s) -
Misiewicz Tracy M.,
Barbosa Carlos E. A.,
Fine Paul V. A.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200229
Subject(s) - biology , burseraceae , microsatellite , amazonian , locus (genetics) , genetic diversity , population , gene flow , allele , botany , ecology , genetics , genetic variation , amazon rainforest , gene , demography , sociology
• Premise of the study: The first microsatellite primers were developed for Protium subserratum , a widespread Amazonian tree, to investigate genetic differentiation between populations found on clay, brown‐sand, and white‐sand soils. • Methods and Results: Seventeen primer pairs were identified from two individuals of P. subserratum found on white‐sand and brown‐sand soil types. Polymorphism was analyzed in 63 individuals from a total of three populations, each found on a different soil type. The primers amplified tetra‐, tri‐, and dinucleotide repeats with three to 24 alleles per locus. Excluding monomorphic loci, observed and expected heterozygosities ranged from 0 to 0.852 and 0.036 to 0.901, respectively. • Conclusions: These new microsatellite markers will be useful in studies of genetic diversity, population differentiation, and gene flow across habitat types in P. subserratum .