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Isolation and characterization of microsatellite markers for an important tropical tree, Aquilaria malaccensis (Thymelaeaceae)
Author(s) -
Tnah Lee Hong,
Lee Chai Ting,
Lee Soon Leong,
Ng Kevin Kit Siong,
Ng Chin Hong,
NurulFarhanah Zakaria,
Lau Kah Hoo,
Chua Lillian Swee Lian
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200165
Subject(s) - biology , microsatellite , genetic diversity , genetic marker , population , thymelaeaceae , loss of heterozygosity , dna profiling , evolutionary biology , genetics , allele , botany , dna , gene , demography , sociology
• Premise of the study: Aggressive collections and trade activities in recent decades have resulted in heavy pressure on the natural stands of Aquilaria malaccensis and concerns over its long‐term survival potential. To aid DNA profiling and assessment of its genetic diversity, microsatellite markers were developed for the species. • Methods and Results: Seventeen polymorphic microsatellite markers were developed for A. malaccensis using an enrichment protocol. The markers were screened on 24 samples from a natural population. The number of alleles ranged from two to 11, and the observed heterozygosity ranged from 0.042 to 0.957. No significant deviation from Hardy–Weinberg equilibrium was detected after conservative Bonferroni correction. • Conclusions: This is the first report on the development of microsatellite markers in A. malaccensis . The markers will be used to establish a DNA profiling database and to estimate the genetic diversity and population genetic structure of the species.