Premium
Characterization of 21 microsatellite markers from cogongrass, Imperata cylindrica (Poaceae), a weed species distributed worldwide
Author(s) -
Chiang YuChung,
Tsai ChiChu,
Hsu TsaiWen,
Chou ChangHung
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200152
Subject(s) - biology , imperata , microsatellite , weed , asexual reproduction , onagraceae , locus (genetics) , loss of heterozygosity , genetic marker , botany , population , allele , genetics , gene , demography , sociology
• Premise of the study: Microsatellite loci were developed from Imperata cylindrica , a traditional medicinal herb in Asia and among the top 10 worst invasive weeds in the world, to aid in the identification of the limits of asexual clonal individuals. • Methods and Results: A total of 21 microsatellite markers, including 18 polymorphic and three monomorphic loci, were developed from I. cylindrica using a magnetic bead enrichment protocol. The primers amplified dinucleotide, trinucleotide, and complex repeats. The number of alleles ranged from one to 19 per locus, with an observed heterozygosity ranging from 0.09 to 1.00. Several loci deviated significantly from the within‐population Hardy–Weinberg equilibrium as a result of asexual clonal reproduction. • Conclusions: These polymorphic markers should be useful tools in further studies on the identification of the range of clonal reproduction units and the selection and classification of the medicinal cultivar.