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Isolation and characterization of 13 novel polymorphic microsatellite markers for Pinus koraiensis (Pinaceae)
Author(s) -
Yu JingHua,
Chen ChangMei,
Tang ZhongHua,
Yuan ShuSheng,
Wang ChunJing,
Zu YuanGang
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200145
Subject(s) - pinus koraiensis , biology , microsatellite , pinaceae , locus (genetics) , allele , loss of heterozygosity , genetic diversity , amplified fragment length polymorphism , genetics , population , evolutionary biology , pinus <genus> , botany , gene , sociology , demography
• Premise of the study: Thirteen polymorphic microsatellite markers were developed to investigate the genetic diversity and population structure of Pinus koraiensis . • Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method with three specific PCR primers for screening the positive clones, 13 loci were found to be polymorphic in 78 individuals of P. koraiensis . Across all of the P. koraiensis samples, the number of alleles per locus ranged from two to 11. • Conclusions: These polymorphic markers will be useful for conservation genetics studies of this species and to inform the development of effective P. koraiensis conservation programs.