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Microsatellite markers for Amborella (Amborellaceae), a monotypic genus endemic to New Caledonia
Author(s) -
Poncet Valérie,
Couderc Marie,
TranchantDubreuil Christine,
Gomez Céline,
Hamon Perla,
Hamon Serge,
Pillon Yohan,
Munzinger Jérôme,
Kochko Alexandre
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200131
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , loss of heterozygosity , allele , population , expressed sequence tag , genetics , evolutionary biology , gene , genome , demography , sociology
• Premise of the study: Informative markers are required for assessing the diversity of Amborella trichopoda , the only species of its order, endemic to New Caledonia and considered to be the sister species to all flowering plants. Therefore, expressed sequence tag (EST)–based microsatellite markers were developed. • Methods and Results: Fifty‐five microsatellite loci were characterized in 14896 putative unigenes, which were generated by assembling A. trichopoda ESTs from the public sequence database. Seventeen markers revealed polymorphism in 80 adult shrubs from three populations. The number of alleles per locus ranged from two to 12, with a total of 132 alleles scored. The mean expected heterozygosity per population ranged from 0.336 to 0.567. • Conclusions: These markers offer an appropriate amount of variation to investigate genetic diversity structure, gene flow, and other conservation issues.