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Development and characterization of nine microsatellite loci for Sisyrinchium micranthum (Iridaceae)
Author(s) -
Tacuatiá Luana O.,
Cidade Fernanda W.,
Souza Anete P.,
SouzaChies Tatiana T.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200105
Subject(s) - biology , microsatellite , locus (genetics) , allele , population , genetics , ploidy , evolutionary biology , gene , demography , sociology
• Premise of the study: Sisyrinchium micranthum is an herb with wide distribution and morphological variation, presenting three known cytotypes (2 x , 4 x , and 6 x ), which is naturally occurring from Mexico to Argentina. The development of microsatellite markers for this species is important to provide molecular tools to investigate polyploidy origin, as well as its relationship with phenotypic and genetic variability. • Methods and Results: Nine microsatellite markers were isolated using an enriched genomic library and characterized in a diploid population. Eight markers generated polymorphic loci in 27 plants of a population from southern Brazil. The number of alleles per locus ranged from two to nine, and the observed and expected heterozygosities varied from 0.192 to 0.741 and from 0.370 to 0.884, respectively. • Conclusions: These species‐specific DNA markers may be useful for further investigations of the involved polyploidization process and the population genetics of S. micranthum .