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Eleven microsatellite markers for the mountain clover Trifolium montanum (Fabaceae)
Author(s) -
Matter Philippe,
Määttänen Kirsti,
Kettle Chris J.,
Ghazoul Jaboury,
Pluess Andrea R.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200102
Subject(s) - biology , microsatellite , pollen , allele , biological dispersal , population , fabaceae , trifolium repens , botany , evolutionary biology , genetics , gene , demography , sociology
• Premise of the study: Microsatellite markers were developed in Trifolium montanum to investigate pollen dispersal patterns at landscape scale with a pollen‐pool analysis (indirect parentage analysis) as well as in an experimental set‐up with a direct paternity analysis. • Methods and Results: Screening 46 microsatellites developed for T. repens yielded four markers usable in T. montanum . Seven additional ones have been developed specifically for the target species using a 454‐sequencing approach. All markers were polymorphic, with an allele number ranging from two to 45 based upon 254 individuals sampled from four populations, and an exclusion probability of 0.999. • Conclusions: These markers proved a useful and reliable molecular tool for use in population genetics and parentage studies of this common grassland herb.