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Development and characterization of 59 polymorphic cDNA‐SSR markers for the edible oil crop Sesamum indicum (Pedaliaceae)
Author(s) -
Wang Linhai,
Zhang Yanxin,
Qi Xiaoqiong,
Gao Yuan,
Zhang Xiurong
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200081
Subject(s) - sesamum , biology , loss of heterozygosity , locus (genetics) , expressed sequence tag , microsatellite , genetics , genetic marker , primer (cosmetics) , allele , germplasm , complementary dna , botany , gene , horticulture , chemistry , organic chemistry
• Premise of the study: Polymorphic simple sequence repeat markers from transcript sequences (cDNA–simple sequence repeat [SSR]) were developed for the edible oil crop Sesamum indicum to facilitate the genetic study of this species. • Methods and Results: We found 7702 SSR loci in the 60960 unigenes, and 1550 primer pairs were designed and synthesized. In total, 59 primer pairs showed polymorphism within 36 individuals; the number of alleles per locus ranged from two to four, and the expected and observed heterozygosity ranged from 0.05 to 0.74 and 0 to 0.30, respectively. • Conclusions: These polymorphic markers will greatly facilitate studies of the genetic structure of S. indicum populations as well as the identification and conservation of the species.