Premium
Microsatellite markers developed for Utricularia reniformis (Lentibulariaceae)
Author(s) -
Clivati Débora,
Gitzendanner Matthew Aaron,
Hilsdorf Alexandre Wagner Silva,
Araújo Welington Luiz,
Oliveira de Miranda Vítor Fernandes
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200080
Subject(s) - biology , microsatellite , genetic diversity , population , botany , allele , evolutionary biology , genetics , gene , demography , sociology
• Premise of the study: Microsatellite markers were developed to study the genetic diversity and population structure of the carnivorous bladderwort Utricularia reniformis , which is endemic to the Atlantic Forest of southern and southeastern Brazil. Cross‐species amplification was tested in U. gibba , U . neottioides , U . subulata , and Pinguicula benedicta . • Methods and Results: The U. reniformis genome was sequenced in a 454 GS FLX sequencer, and eight primer sets were developed based on the microsatellites identified from the reads. All loci are polymorphic, showing 1.6 to 4.8 alleles per population. Preliminary results show that primer sets are suitable for population‐level studies. Cross‐species amplification was successful in three other Utricularia species and one Pinguicula species. • Conclusions: Markers developed in this study provide tools for analyses of intra‐ and interpopulation genetic diversity in Utricularia and Pinguicula .