Premium
Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America
Author(s) -
Barrandeguy María E.,
Prinz Kathleen,
García María V.,
Finkeldey Reiner
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200078
Subject(s) - biology , microsatellite , fabaceae , genetic diversity , locus (genetics) , genetics , allele , genetic marker , genetic structure , evolutionary biology , botany , genetic variation , population , gene , demography , sociology
• Premise of the study: Microsatellite primers were developed in the native legume tree Anadenanthera colubrina var. cebil to study the genetic diversity and genetic structure in natural populations in Argentina. • Methods and Results: Nine microsatellite markers were identified using a genomic library enriched for tandemly repeated motifs, eight of which markers were polymorphic. The polymorphism of these markers was assessed by investigating 20 individuals for fragment polymorphism; three to 13 alleles were observed for each locus. Observed and expected heterozygosities ranged from 0.300 to 1.000 and from 0.463 to 0.900, respectively. • Conclusions: These results confirm that these primers will be useful for investigating the genetic diversity and genetic structure of natural populations of A. colubrina var. cebil in future studies.