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Development of microsatellite markers from Cercis chinensis (Fabaceae)
Author(s) -
Gong Wei,
Duan Tingting,
Zhang Dianxiang
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200065
Subject(s) - biology , microsatellite , amplified fragment length polymorphism , genetic diversity , genetic structure , phylogeography , fabaceae , population , evolutionary biology , botany , allele , genetics , genetic variation , phylogenetics , gene , demography , sociology
• Premise of the study: Microsatellite markers were developed to characterize the level of genetic diversity and population genetic structure of Cercis chinensis , a widely cultivated garden plant in China with congeneric species disjunctly distributed in East Asia, North America, and the Mediterranean. • Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, eight microsatellite markers were developed in C. chinensis . Seven of the markers displayed polymorphism, with the number of alleles ranging from one to four in four populations of C. chinensis . Four to six microsatellite loci exhibited interspecific transferability in C. glabra , C. chuniana , and C. chingii . • Conclusions: These are the first microsatellite markers developed for C. chinensis , which will be further used in investigation of population genetic structure and phylogeographic pattern of C. chinensis and its congeneric species.