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Characterization of new microsatellite loci isolated from Santiria trimera (Burseraceae)
Author(s) -
Koffi K. Guillaume,
Heuertz Myriam,
Jans Romain,
Hardy Olivier J.,
Vendramin Giovanni G.,
Duminil Jérôme
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200041
Subject(s) - biology , microsatellite , sympatric speciation , allele , genetics , genetic diversity , genetic structure , pyrosequencing , evolutionary biology , genetic variation , gene , population , demography , sociology
• Premise of the study: To study the genetic structure among three morphotypes of an African rainforest tree species, Santiria trimera , nuclear microsatellite markers were isolated and characterized. • Methods and Results: Seven polymorphic loci were isolated using a pyrosequencing‐based protocol and successfully amplified on three different morphotypes of S. trimera . For six of the seven loci, there is at least one private allele for one of the three morphotypes. The mean effective number of alleles is about four for each of the three morphotypes. • Conclusions: These microsatellite markers are promising to explore the genetic delimitation among sympatric morphotypes found in Gabonese forests and to study the spatial genetic structure within each gene pool.