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Microsatellite primers in Oenothera harringtonii (Onagraceae), an annual endemic to the shortgrass prairie of Colorado
Author(s) -
Skogen Krissa A.,
Hilpman Evan T.,
Todd Sadie L.,
Fant Jeremie B.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200003
Subject(s) - biology , microsatellite , onagraceae , gene flow , oenothera , genetic diversity , locus (genetics) , allele , botany , taxon , genetic variation , genetics , gene , population , demography , sociology
• Premise of the study: Microsatellite markers were developed in the annual herb, Oenothera harringtonii , to investigate patterns of genetic diversity, gene flow, and parentage within and among populations of this Colorado endemic. • Methods and Results: Ten polymorphic loci were identified in O. harringtonii and tested in four populations sampled across the range of the species. These loci contained trinucleotide repeats with 7–29 alleles per locus. Nine of the 10 loci also amplified in O. caespitosa subsp. macroglottis , O. caespitosa subsp. marginata , and O. caespitosa subsp. navajoensis . In addition, we optimized three markers developed for O. biennis and provide reports of their effectiveness in all four taxa. • Conclusions: These results indicate the utility of these markers in O. harringtonii for future studies of genetic structure, gene flow, and parentage as well as their applicability in other members of the O. caespitosa species complex.