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Microsatellite loci in Ipomopsis aggregata (Polemoniaceae) and cross‐species applicability for ecological genetics studies
Author(s) -
Wu Carrie A.,
Smith Clifford J.,
Massaro Andrew J.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100612
Subject(s) - biology , ecological genetics , evolutionary biology , microsatellite , ecology , zoology , genetics , gene , allele , population , demography , sociology
• Premise of the study: Novel microsatellite primers were developed for the native wildflower Ipomopsis aggregata to facilitate ongoing studies of the genetics of local adaptation and patterns of hybridization with closely related species within the genus. • Methods and Results: Thirteen primer sets were successfully developed and tested using populations from the Rocky Mountains of Colorado, USA. The primers amplified di‐, tri‐, and tetranucleotide repeats with 1–15 alleles per locus. All primers also amplified fragments with varying success in closely related Ipomopsis species and more distant members of the Polemoniaceae. • Conclusions: The polymorphism levels observed across all loci suggest that these microsatellites may be useful for population genetic studies in Ipomopsis , as well as in studies of other related taxa in the Polemoniaceae.