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Microsatellite markers for Vellozia gigantea (Velloziaceae), a narrowly endemic species to the Brazilian campos rupestres
Author(s) -
Martins Ana Paula V.,
Proite Karina,
Kalapothakis Evanguedes,
Santos Fabrício R.,
Chaves Anderson V.,
Borba Eduardo L.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100611
Subject(s) - gigantea , biology , microsatellite , loss of heterozygosity , locus (genetics) , allele , genetics , endemism , endangered species , population , botany , gene , ecology , habitat , demography , sociology
• Premise of the study: Microsatellite primers were developed for the first time in Velloziaceae, in the endangered species Vellozia gigantea . • Methods and Results: Using two different protocols, seven primer sets were characterized in three populations of V. gigantea . The primers amplified di‐ and trinucleotide repeats with six to 12 alleles per locus. These revealed high levels of genetic variation, presenting an average observed heterozygosity of 0.508 in V. gigantea . The seven primers were tested for cross‐amplification in three Vellozia species. All primers successfully amplified in V. auriculata . Six primers amplified in V. compacta and three in V. hirsuta . • Conclusions: The new marker set described here will be useful for studies of population genetics of V. gigantea . The cross‐amplification results indicate the utility of primers for studies in other Vellozia species.