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Microsatellite markers for Duperrea pavettifolia (Rubiaceae)
Author(s) -
Lin Hua,
Gao Jiangyun,
Zhang Zhirong,
Fan Xuli,
Zhou Xiang
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100609
Subject(s) - biology , microsatellite , genetics , amplified fragment length polymorphism , population , evolutionary biology , mating , mating system , population genetics , genetic marker , genetic diversity , gene , allele , demography , sociology
• Premise of the study: The development of microsatellite primers for Duperrea pavettifolia will be the foundation for mating system analysis and conservation research. • Methods and Results: Nineteen microsatellite markers were developed and characterized in two wild populations by using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Polymorphisms were evaluated in 24 individuals from two natural populations. Eleven of these primers generated polymorphic loci. • Conclusions: These microsatellite markers will be useful in future investigations into the population genetics and mating system of D. pavettifolia .