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Microsatellite loci for tucumã of Amazonas ( Astrocaryum aculeatum ) and amplification in other Arecaceae
Author(s) -
Ramos Santiago L. Ferreyra,
Macêdo Jeferson L. Vasconcelos,
Lopes Maria T. Gomes,
Batista Jacqueline S.,
Formiga Kyara M.,
Silva Perla Pimentel,
SauloMachado Antonio C.,
Veasey Elizabeth Ann
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100607
Subject(s) - biology , arecaceae , microsatellite , genetic diversity , population , genetics , allele , evolutionary biology , palm , gene , demography , physics , quantum mechanics , sociology
• Premise of the study: Microsatellite loci were developed for tucumã of Amazonas ( Astrocaryum aculeatum ), and cross‐species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management. • Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite‐enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae. • Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã.