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Development of microsatellite markers in a riparian shrub, Spiraea thunbergii (Rosaceae)
Author(s) -
Ashizawa Kazuya,
Kimura Megumi K.,
Takahashi Akiko,
Lian Chunlan,
Kuramoto Noboru
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100587
Subject(s) - biology , microsatellite , shrub , pinus thunbergii , genetic diversity , population , locus (genetics) , botany , amplified fragment length polymorphism , genetic marker , primer (cosmetics) , genetics , allele , gene , demography , sociology , chemistry , organic chemistry
• Premise of the study: Microsatellite primers in the deciduous shrub Spiraea thunbergii were developed to investigate genetic diversity and population genetic structure. Cross‐species transferability was assayed in four congeneric species. • Methods and Results: Using a compound simple sequence repeat (SSR) marker method, 10 primer sets were identified in Japanese populations of S. thunbergii . The primers amplified compound SSRs with two to five alleles per locus. More than half of the primers were also amplified in S. prunifolia , S. nipponica var. nipponica , and S. japonica . • Conclusions: These markers might be useful for future studies of population genetics of S. thunbergii and congeneric species.