Premium
Development of 12 microsatellite markers for Platycrater arguta (Hydrangeaceae) endemic to East Asia
Author(s) -
Qi Xinshuai,
Yuan Na,
Qiu YingXiong
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100582
Subject(s) - microsatellite , biology , phylogeography , amplified fragment length polymorphism , genetics , allele , locus (genetics) , evolutionary biology , population , genetic structure , population genetics , genetic marker , genetic diversity , genetic variation , phylogenetics , gene , demography , sociology
• Premise of the study: Microsatellite markers were characterized in Platycrater arguta , a rare endemic shrub from eastern China and southern Japan, to investigate its population genetic structure, phylogeographic patterns, and breeding system. • Methods and Results: Using both the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol and the compound microsatellite marker technique, we developed 12 microsatellite markers. All primers showed polymorphism when assessed in 43 individuals from two populations in eastern China and southern Japan. Overall, the number of alleles ranged from five to 21, with an average of 14.91 alleles per locus. • Conclusions: These markers can be used in further studies on population genetics, phylogeography, and breeding system of P. arguta .