Isolation and characteristics of eight novel polymorphic microsatellite loci in  Lippia alba  (Verbenaceae) | Zendy

Santos Fernanda R. C. | Zendy; Lima Paula F. | Zendy; Priolli Regina H. G. | Zendy; Siqueira Walter J. | Zendy; Colombo Carlos A. | Zendy

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Isolation and characteristics of eight novel polymorphic microsatellite loci in Lippia alba (Verbenaceae)

Author(s) -

Santos Fernanda R. C.,

Lima Paula F.,

Priolli Regina H. G.,

Siqueira Walter J.,

Colombo Carlos A.

Publication year - 2012

Publication title -

american journal of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.218

H-Index - 151

eISSN - 1537-2197

pISSN - 0002-9122

DOI - 10.3732/ajb.1100578

Subject(s) - biology , microsatellite , verbenaceae , loss of heterozygosity , allele , genetic diversity , genetics , evolutionary biology , botany , population , gene , demography , sociology

• Premise of the study: A set of eight microsatellite (simple sequence repeat [SSR]) markers for Lippia alba , an important medicinal and cosmetic plant, was developed to aid studies of genetic diversity and to define efficient strategies for breeding programs. • Methods and Results: Using a (CT) 8 ‐ and (GT) 8 ‐enriched library, a total of 11 SSR loci were developed and optimized in L. alba . Of the 11 loci, eight were found to be polymorphic after screening 61 accessions from two populations. The parameters used to characterize loci were expected heterozygosity ( H e ) and number of alleles. A total of 44 alleles were identified, with an average of 5.5 alleles per loci, which were moderately to highly informative according to H e . • Conclusions: These new SSR markers have potential for informing genetic diversity, allele mining, and mapping studies and will be used to generate information for breeding programs of L. alba .

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