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Isolation and characterization of microsatellite markers from Clematoclethra scandens (Actinidiaceae)
Author(s) -
Li Lu,
Chen Li,
Zhang ZhiRong,
Xiao LongQian
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100556
Subject(s) - microsatellite , biology , amplified fragment length polymorphism , genetics , evolutionary biology , genetic algorithm , population , population genetics , allele , genetic diversity , gene , demography , sociology
• Premise of the study: Microsatellite makers were developed for Clematoclethra scandens to investigate its population genetics and speciation. • Methods and Results: A total of 36 microsatellite markers were isolated using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method. Their polymorphisms were assessed in two natural populations. The results showed that 30 markers displayed prominent polymorphisms and six markers were monomorphic. • Conclusions: These microsatellite loci will facilitate further studies on population genetics and speciation of C. scandens .