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Microsatellite markers for Grosmannia alacris (Ophiostomataceae, Ascomycota) and other species in the G. serpens complex
Author(s) -
Duong Tuan A.,
Beer Z. Wilhelm,
Zanzot James W.,
Wingfield Michael J.,
Wingfield Brenda D.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100527
Subject(s) - biology , microsatellite , ascomycota , primer (cosmetics) , population , genetics , genetic marker , serpens , evolutionary biology , gene , allele , chemistry , demography , star formation , stars , organic chemistry , physics , astronomy , sociology
• Premise of the study: Polymorphic microsatellite markers were developed for the pine‐infecting fungus, Grosmannia alacris . • Methods and Results: Sixteen microsatellite markers were developed by using inter‐simple sequence repeat (ISSR)–PCRs and 454 sequencing methods. Seven of these markers showed polymorphisms for a South African population of G. alacris , and 13 markers showed polymorphism when European isolates were included. Most of the primer pairs also amplified four closely related species: G. serpens , Leptographium gibbsii , L. castellanum , and L. yamaokae . • Conclusions: These new markers will be useful for population studies of G. alacris and other species in the G. serpens complex.