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Development of novel chloroplast microsatellite markers for Miscanthus species (Poaceae)
Author(s) -
Jiang JianXiong,
Wang ZuHong,
Tang BingRong,
Xiao Liang,
Ai Xin,
Yi ZiLi
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100518
Subject(s) - miscanthus , biology , microsatellite , locus (genetics) , miscanthus sinensis , genetics , allele , saccharum officinarum , poaceae , genetic diversity , botany , population , ecology , gene , bioenergy , demography , sociology , renewable energy
• Premise of the study: A set of novel chloroplast microsatellite markers (cpSSRs) was developed for the bioenergy crop Miscanthus , and their utility in cross‐species amplification was evaluated. • Methods and Results: Twenty‐eight novel primers flanking cpSSR loci were designed from a complete chloroplast genome sequence of Saccharum officinarum , a species closely related to Miscanthus . These primers were then tested on eight Miscanthus species, among which 16 cpSSR loci were found to be polymorphic. The number of alleles per polymorphic locus ranged from two to seven, with an average 3.94 alleles. • Conclusions: These cpSSR markers can be applied to all Miscanthus species and will be useful for studying Miscanthus population structure, diversity, and phylogeography.