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Development and characterization of 38 polymorphic microsatellite markers from an economically important fruit tree, the Indian jujube
Author(s) -
Chiou ChuYing,
Chiang YuChung,
Chen ChihHsiung,
Yen ChungRuey,
Lee SheueRu,
Lin YuShium,
Tsai ChiChu
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100500
Subject(s) - biology , microsatellite , ziziphus , genetic diversity , ziziphus jujuba , cultivar , loss of heterozygosity , allele , genetic relationship , polymorphism (computer science) , genetic marker , botany , horticulture , genetics , gene , population , demography , sociology
• Premise of the study: A total of 38 polymorphic microsatellite loci of the Indian jujube ( Ziziphus mauritiana ), an economically important fruit tree, were developed to evaluate genetic diversity and aid in the identification of cultivars. • Methods and Results: The 38 microsatellite markers were isolated from the Indian jujube using a magnetic bead enrichment method, and polymorphisms were identified in 24 Indian jujube cultivars. The number of alleles ranged from two to 13, with expected heterozygosity ranging from 0.261 to 0.898. The polymorphism information content ranged from 0.248 to 0.889, with a mean of 0.616. Of these 38 simple sequence repeat loci, 20 loci from Z. jujuba (Chinese jujube) were successfully amplified using the simple sequence repeat primer sets. • Conclusions: These polymorphic loci should be useful in further studies of the genetic diversity and the identification of cultivars of both the Indian jujube and the Chinese jujube.