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Development of PCR primer sets for intron 1 of the low‐copy gene LEAFY in Davalliaceae
Author(s) -
Chen ChengWei,
Kuo LiYaung,
Wang ChunNeng,
Chiou WenLiang
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100498
Subject(s) - biology , leafy , intron , genetics , intergenic region , phylogenetic tree , primer (cosmetics) , gene , botany , genome , chemistry , organic chemistry
• Premise of the study: Primers were designed for amplifying intron 1 of the single‐copy nuclear LEAFY gene for species of Davalliaceae. • Methods and Results: New primer sets were designed and successfully amplified for intron 1 of the LEAFY gene in 13 species representing the five genera of Davalliaceae. The orthology of these sequences was further confirmed by phylogenetic analyses. Site variation in LEAFY intron 1 sequences across genera of the Davalliaceae and among accessions of the Humata repens complex were 18% and 8%, respectively. Such variation was greater than that for the cpDNA atpB‐rbcL intergenic spacer region across the same taxa and accessions. • Conclusions: Using our newly designed primers, intron 1 of the LEAFY gene could be amplified for all species tested. In addition, this single‐copy, biparentally inherited, and quickly evolving region showed considerable potential for addressing infraspecific‐level questions.