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Development of polymorphic microsatellite markers in Camellia chekiangoleosa (Theaceae) using 454‐ESTs
Author(s) -
Wen Qiang,
Xu Linchu,
Gu Yincong,
Huang Minren,
Xu Lian
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100486
Subject(s) - biology , microsatellite , germplasm , camellia , genetic diversity , allele , genetics , theaceae , population , evolutionary biology , camellia sinensis , botany , gene , demography , sociology
• Premise of the study: A set of microsatellite markers for Camellia chekiangoleosa was developed and characterized using 454 sequencing technology to study the population genetic structure and the diversity of germplasm collections. • Methods and Results: Eighteen polymorphic microsatellite markers were identified and tested in 150 individuals from three natural populations of C. chekiangoleosa . Alleles numbered from two to seven, and the observed and expected heterozygosities ranged from 0.100 to 0.760 and 0.133 to 0.809, respectively. • Conclusions: These markers will potentially be conducive to further genetic studies on C. chekiangoleosa .