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Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing
Author(s) -
Wöhrmann Tina,
Wagner Natascha,
Krapp Florian,
Huettel Bruno,
Weising Kurt
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100470
Subject(s) - biology , microsatellite , pyrosequencing , genetic diversity , genetics , allele , population , primer (cosmetics) , genetic marker , genotype , evolutionary biology , gene , chemistry , demography , organic chemistry , sociology
• Premise of the study: Polymorphic microsatellite markers were developed for Fosterella rusbyi (Bromeliaceae) to evaluate the population genetic structure and genetic diversity of natural populations of F. rusbyi and other Fosterella species in Bolivia. • Methods and Results: 454 pyrosequencing technology was used to generate 73027 sequence reads from F. rusbyi DNA, which together contained 2796 perfect simple sequence repeats (SSRs). Primer pairs were designed for 30 loci, of which 15 were used to genotype 30 F. rusbyi plants from two geographical areas in Bolivia. All markers were polymorphic, with two to nine alleles in the overall sample. Cross‐species amplification was tested in 10 additional Fosterella species. Seven loci showed consistent amplification in six or more species. • Conclusions: The 15 SSR markers developed for F. rusbyi are promising candidates for population genetic analyses within F. rusbyi and other species of Fosterella .