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Microsatellite markers from Ceanothus roderickii (Rhamnaceae) using next‐generation sequencing technology
Author(s) -
Burge Dylan O.,
Jui Ginger,
Kembel Steven W.,
Zhukovsky Katherine
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100431
Subject(s) - biology , microsatellite , locus (genetics) , genetics , gene flow , rhamnaceae , population , population genetics , loss of heterozygosity , evolutionary biology , allele , genetic variation , ecology , gene , demography , sociology
• Premise of the study: Ceanothus roderickii is an endangered shrub endemic to California. To investigate the population genetics of this species, including the genetic consequences of population fragmentation and hybridization, 10 microsatellite markers were developed. • Methods and Results: Using next‐generation sequencing (454) data from a single C. roderickii individual, 10 microsatellite markers were developed. A group of 12 individuals representing all of the major C. roderickii populations were analyzed. All loci were found to be polymorphic, with a range from two to 12 alleles per locus. Observed heterozygosity ranged from 0.08 to 0.83 across loci. All 10 loci were also amplifiable in at least one other Ceanothus species. • Conclusions: Results presented here indicate the utility of our new microsatellite primers in ongoing and future studies concerning population genetics and gene flow in C. roderickii , as well as the potential applicability of these primers in similar studies on other Ceanothus species.