z-logo
Premium
Microsatellite markers for the native Texas perennial grass, Panicum hallii (Poaceae)
Author(s) -
Lowry David B.,
Purmal Colin T.,
Meyer Eli,
Juenger Thomas E.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100430
Subject(s) - biology , panicum , poaceae , perennial plant , microsatellite , panicum virgatum , botany , ecology , genetics , gene , allele , bioenergy , renewable energy
• Premise of the study: We developed microsatellites for Panicum hallii for studies of gene flow, population structure, breeding experiments, and genetic mapping. • Methods and Results: Next‐generation (454) genomic sequence data were used to design markers. Eighteen robust markers were discovered, 15 of which were polymorphic across six accessions of P. hallii var. hallii . Fourteen of the markers cross‐amplified in a P. capillare accession. For the 15 polymorphic markers, the total number of alleles per locus ranged from two to 26 (mean: 11.0) across six populations (11–19 individuals per population). Observed heterozygosity (mean: 0.031) was 13.7 times lower than the expected heterozygosity (mean: 0.426). • Conclusions: The deficit of heterozygous individuals is consistent with P. hallii having a high rate of self‐fertilization. These markers will be useful for studies in P. hallii and related species.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here