Premium
Microsatellite markers for the native Texas perennial grass, Panicum hallii (Poaceae)
Author(s) -
Lowry David B.,
Purmal Colin T.,
Meyer Eli,
Juenger Thomas E.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100430
Subject(s) - biology , panicum , poaceae , perennial plant , microsatellite , panicum virgatum , botany , ecology , genetics , gene , allele , bioenergy , renewable energy
• Premise of the study: We developed microsatellites for Panicum hallii for studies of gene flow, population structure, breeding experiments, and genetic mapping. • Methods and Results: Next‐generation (454) genomic sequence data were used to design markers. Eighteen robust markers were discovered, 15 of which were polymorphic across six accessions of P. hallii var. hallii . Fourteen of the markers cross‐amplified in a P. capillare accession. For the 15 polymorphic markers, the total number of alleles per locus ranged from two to 26 (mean: 11.0) across six populations (11–19 individuals per population). Observed heterozygosity (mean: 0.031) was 13.7 times lower than the expected heterozygosity (mean: 0.426). • Conclusions: The deficit of heterozygous individuals is consistent with P. hallii having a high rate of self‐fertilization. These markers will be useful for studies in P. hallii and related species.