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Development of 15 novel microsatellite markers from cellulose synthase genes in Populus tomentosa (Salicaceae)
Author(s) -
Du QingZhang,
Zhang DeQiang,
Li BaiLian
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100308
Subject(s) - biology , microsatellite , salicaceae , loss of heterozygosity , locus (genetics) , germplasm , allele , genetic diversity , gene , genetic marker , genetics , ploidy , botany , cellulose , woody plant , biochemistry , population , demography , sociology
• Premise of the study: Microsatellite markers from cellulose synthase genes were developed for the Chinese white poplar, Populus tomentosa , to investigate the genetic diversity of wild germplasm resources and to further identify favorable alleles significantly associated with wood cellulose content. • Methods and Results: Fifteen microsatellite markers were developed in P. tomentosa by deep sequencing of cellulose synthase genes. Polymorphisms were evaluated in 460 individuals from three climatic regions of P. tomentosa , and all 15 markers revealed polymorphic variation. The number of alleles per locus ranged from two to nine with an average of 4.3; the observed and expected heterozygosity per locus varied from 0.029 to 0.962 and from 0.051 to 0.713, respectively. • Conclusions: These polymorphic markers will potentially be useful for genetic mapping and in molecular breeding for improvement of wood fiber traits in Populus .