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Characterization of 24 transferable microsatellite loci in four skullcaps ( Scutellaria , Labiatae)
Author(s) -
Chiang YuChung,
Huang BingHong,
Shih HueiChuan,
Hsu TsaiWen,
Chang ChunWen,
Liao PeiChun
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100279
Subject(s) - biology , microsatellite , scutellaria , genetics , loss of heterozygosity , population , allele , evolutionary biology , botany , gene , medicine , pathology , sociology , alternative medicine , demography , traditional chinese medicine
• Premise of the study: Transferable polymorphic microsatellite loci for four skullcaps, Scutellaria indica , S. taiwanensis , S. austrotaiwanensis , and S. playfairii , were developed for future studies of the mating system and population structure of these species. Interspecific amplification was also tested in various Scutellaria species. • Methods and Results: Twelve novel polymorphic microsatellite loci were isolated from four S. taiwanensis specimens, and seven are interspecifically transferable. Microsatellite loci developed from S. austrotaiwanensis in a previous study were also analyzed in the other three species, and 12 loci were found to be transferable. Allele numbers of the total 24 loci for S. indica , S. taiwanensis , S. playfairii , and S. austrotaiwanensis are two to four, two, two to five, and two to three, respectively, with an expected heterozygosity ranging from 0.114–0.661, 0.062–0.499, 0.280–0.730, and 0.268–0.662, respectively. The interspecies transferability of these 24 loci was further tested in another 10 Scutellaria species, including three species native to Taiwan. Seventeen loci were found to be interspecifically amplifiable, especially among the Taiwan native species. • Conclusions: These highly polymorphic and transferable loci will be useful for future studies of the mating system of closely related Scutellaria species.