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Characterization of microsatellite loci in Festuca gautieri (Poaceae) and transferability to F. eskia and F. ×picoeuropeana
Author(s) -
SegarraMoragues José Gabriel,
Catalán Pilar
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100267
Subject(s) - biology , microsatellite , genetic diversity , transferability , allele , festuca , population , population genetics , loss of heterozygosity , range (aeronautics) , poaceae , botany , genetics , demography , statistics , mathematics , logit , sociology , gene , materials science , composite material
• Premise of the study: Enriched genomic libraries were used to isolate and characterize microsatellite loci in Festuca gautieri , an important plant component of subalpine calcareous grasslands of the eastern Iberian Peninsula, the Pyrenees, and the Cantabrian Mountains. Microsatellites were required to investigate landscape genetics across its distribution range and at a narrower geographical scale within the Ordesa y Monte Perdido, Aigüestortes, and Picos de Europa Spanish national parks. • Methods and Results: Ten polymorphic microsatellite loci were characterized. They amplified a total of 116 alleles in a sample of 30 individuals of F. gautieri , showing high levels of genetic diversity (expected heterozygosity = 0.821). Cross‐species transferability to two other close congeners, F. eskia and F ×picoeuropeana, increased the total number of alleles to 137. These taxa showed lower numbers of alleles but similar levels of genetic diversity to F. gautieri . • Conclusions: These microsatellite primers will be useful in population and landscape genetics and in establishing conservation strategies for these characteristic elements of subalpine pastures.