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Nuclear and chloroplast SSR markers in Paeonia delavayi (Paeoniaceae) and cross‐species amplification in P. ludlowii
Author(s) -
Zhang JinMei,
Liu Jing,
Sun HaiLu,
Yu Jing,
Wang JianXiu,
Zhou ShiLiang
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100240
Subject(s) - biology , microsatellite , locus (genetics) , genetic diversity , allele , genetics , nuclear gene , population , chloroplast dna , botany , chloroplast , genome , gene , demography , sociology
• Premise of the study: Microsatellite primers were developed for Paeonia delavayi and P. ludlowii (Paeoniaceae) to study their population genetics and phytogeography. • Methods and Results: Nine polymorphic nuclear microsatellite loci were isolated from an enriched library of P. delavayi and primers were designed. The number of alleles per locus ranged from two to 16; the observed and expected heterozygosities ranged from 0.014 to 0.687 and 0.042 to 0.875, respectively. Six polymorphic chloroplast microsatellite loci were identified in P. delavayi and primers were provided. The number of alleles per locus ranged from two to six and the polymorphic information content ranged from 0.08 to 0.716. Both nuclear and chloroplast primers were successfully applicable to P. ludlowii . • Conclusions: The markers developed here will facilitate analyses of genetic diversity, population genetic structure, phytogeographical patterns, and conservation for P. delavayi and P. ludlowii .