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Characterization of long transcribed microsatellites in Helianthus annuus (Asteraceae)
Author(s) -
Pramod Sreepriya,
Rasberry Armed B.,
Butler Tiffini G.,
Welch Mark E.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100232
Subject(s) - microsatellite , biology , loss of heterozygosity , genetics , locus (genetics) , allele , internal transcribed spacer , helianthus , evolutionary biology , helianthus annuus , asteraceae , population , selection (genetic algorithm) , botany , phylogenetic tree , gene , sunflower , demography , artificial intelligence , sociology , computer science , agronomy
• Premise of the study: Research on the evolutionary role of exonic microsatellites currently lacks an understanding of the evolutionary pressures that promote or limit their expansion. Contrasting levels of variability and genetic structures at anonymous and transcribed microsatellite loci of varying lengths are likely to provide useful insights regarding the relative strength of selection acting on different classes of microsatellites. We have developed primers for long transcribed microsatellites in Helianthus annuus to make these comparisons. • Methods and Results: Eight relatively long microsatellites from sequences in the expressed sequence tag database of H. annuus were characterized. A total of 63 individuals from three populations in Kansas were genotyped. The number of alleles per locus ranged from four to 11 with an average observed heterozygosity of 0.723. • Conclusions: Our study has generated suitable tools for studying the population genetics of long transcribed microsatellites that are potentially influenced by selection.