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Single nucleotide polymorphism marker development in the rubber tree, Hevea brasiliensis (Euphorbiaceae)
Author(s) -
Pootakham Wirulda,
Chanprasert Juntima,
Jomchai Nukoon,
Sangsrakru Duangjai,
Yoocha Thippawan,
Therawattanasuk Kanikar,
Tangphatsornruang Sithichoke
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100228
Subject(s) - hevea brasiliensis , biology , single nucleotide polymorphism , snp , genetics , loss of heterozygosity , hevea , computational biology , allele , genotype , gene , natural rubber , chemistry , organic chemistry
• Premise of the study: We demonstrated the application of high‐throughput 454 sequencing technology in the identification of single nucleotide polymorphism (SNP) markers in Hevea brasiliensis . • Methods and Results: A total of 5883 putative SNP positions were discovered in silico, and 10 biallelic SNP markers were validated from 454‐derived EST sequences. The polymorphism information content ( PIC ) and the observed heterozygosity ( H o ) ranged from 0.0963–0.5135 and 0.1071–0.4643, respectively. • Conclusions: These markers can be useful for the construction of genetic maps, the identification of quantitative trait loci linked to commercially desirable traits, and the study of genetic structure in H. brasiliensis .