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Isolation and characterization of microsatellite markers for Dipteronia dyerana (Sapindaceae), an endangered endemic species in China
Author(s) -
Chen Chen,
Ren BingBing,
Xu XiHui,
Fu ChengXin,
Qiu YingXiong
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100185
Subject(s) - biology , microsatellite , endangered species , amplified fragment length polymorphism , locus (genetics) , genetics , population , allele , population genetics , genetic marker , evolutionary biology , zoology , genetic diversity , ecology , gene , demography , sociology , habitat
• Premise of the study: Microsatellite markers were developed for Dipteronia dyerana , an endangered endemic species in China, to study the population genetics of this species. • Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 12 microsatellite markers that were successfully amplified showed polymorphism when tested on 31 individuals from two populations in the counties of Wenshan and Mengzi. Overall, the number of alleles per locus ranged between seven and 25, with an average of 12.3. Nine of these markers were able to be amplified in D. sinensis . • Conclusions: These microsatellite markers should facilitate further studies on the population genetics and the reproductive ecology of Dipteronia .