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Isolation, characterization, and cross‐amplification of microsatellite markers for the Petunia integrifolia (Solanaceae) complex
Author(s) -
Kriedt Raquel A.,
RamosFregonezi Aline M. C.,
Beheregaray Luciano B.,
Bonatto Sandro L.,
Freitas Loreta B.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100178
Subject(s) - biology , microsatellite , petunia , introgression , genotyping , genetics , botany , genetic marker , plant genetics , hybrid , allele , genotype , genome , gene
• Premise of the study: Microsatellite markers were developed for Petunia integrifolia subsp. depauperata with an intent to clarify taxonomic questions on the P. integrifolia complex, and to identify a purple‐flowered parent of P. hybrida . • Methods and Results: We characterized 11 microsatellite loci by screening primers developed using an SSR‐enriched library. Genotyping of two populations resulted in eight polymorphic loci. Cross‐species transferability was tested for other members of the P. integrifolia complex. • Conclusions: The development of these markers may contribute to population genetics studies in Petunia , and cross‐amplification among related species could be a useful tool for research on hybridization and introgression.