Premium
Rapid isolation and cross‐amplification of microsatellite markers in Plectritis congesta (Valerianaceae) with 454 sequencing
Author(s) -
McEwen Jamie R.,
Vamosi Jana C.,
Rogers Sean M.
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100160
Subject(s) - biology , microsatellite , genetics , gene duplication , loss of heterozygosity , allele , genetic marker , evolutionary biology , gene
• Premise of the study: Microsatellite markers were isolated and characterized in Plectritis congesta for studying the evolution of this highly variable species. • Methods and Results: We used 454 sequencing of DNA enriched for microsatellite repeats to develop microsatellite markers. This produced 262079 reads with an average length of 324 bp, representing approximately 800 microsatellite regions from which 48 primers were tested. Eleven markers reliably amplified without optimization. These primer pairs showed a high degree of heterozygosity and allelic diversity. Unexpectedly, half of the markers contained multiple peaks, with up to four alleles per individual, which suggests that either polyploidy or isolated gene duplication has occurred within this clade. These primers successfully cross‐amplified in P. macrocera , indicating the utility of these markers for the genus. • Conclusions: With variation in mating system and habitat, a mix of duplicated and nonduplicated markers, and high genetic variance, Plectritis is an ideal candidate model genus for studying the ecological and evolutionary consequences of gene duplication.