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Microsatellite marker development in tung trees ( Vernicia montana and V. fordii , Euphorbiaceae)
Author(s) -
Xu Wei,
Yang Qing,
Huai Huyin,
Liu Aizhong
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100151
Subject(s) - biology , microsatellite , genetic diversity , euphorbiaceae , botany , genetic marker , population , amplified fragment length polymorphism , genetics , allele , gene , demography , sociology
• Premise of the study: The tung tree is valuable for tung oil, which is derived from the oilseeds of the tree. Microsatellite markers were developed in tung trees ( Vernicia montana and V. fordii ) for use in population genetic studies and, particularly, analyses of genetic relationships among germplasms. • Methods and Results: A total of 20 microsatellite markers were developed and characterized in V. montana and tested for cross‐amplification in V. fordii , using the Fast Isolation by AFLP of Sequences COntaining Repeats protocol. These markers showed rich polymorphism when assessed in 120 samples of V. montana and six samples of V. fordii . • Conclusions: The developed microsatellite markers will be of great use for investigating genetic diversity of germplasms, analyzing population structure, and facilitating molecular breeding in tung trees.