Premium
Characterization of nuclear microsatellite loci in the calcareous fen specialist Scorpidium cossonii (Calliergonaceae)
Author(s) -
Kophimai Yuppayao,
Cornejo Carolina,
Peintinger Markus,
Bergamini Ariel,
Scheidegger Christoph
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100144
Subject(s) - biology , microsatellite , genetic diversity , locus (genetics) , gene flow , allele , genetic structure , genetics , genetic marker , evolutionary biology , genetic variation , gene , population , demography , sociology
• Premise of the study: Nuclear microsatellite markers were developed in the fen specialist moss, Scorpidium cossonii , to study genetic diversity and genetic structure of this species in relation to land‐use types, habitat fragmentation, and habitat conservation measures. • Methods and Results: The polymorphisms of 14 microsatellite markers were characterized. All primers were tested on 140 gametophytes collected from four populations in Switzerland. The primers amplified di‐ and trinucleotide repeats with three to 26 alleles per locus. The primers worked also in the two other species of the genus: 14 and 12 primers successfully amplified in S. revolvens and S. scorpioides , respectively. • Conclusions: In future studies, our primers have the potential to provide valuable information on genetic diversity, genetic structure, and on historical and recent gene flow in S . cossonii ; they should also enable related research in other Scorpidium species.