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Development and characterization of microsatellite markers in the critically endangered species Acer yangbiense (Aceraceae)
Author(s) -
Zhao Linlin,
Sun Weibang,
Yang Junbo
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100142
Subject(s) - microsatellite , biology , genetic diversity , critically endangered , locus (genetics) , endangered species , genetics , population , allele , evolutionary biology , ecology , gene , demography , sociology , habitat
• Premise of the study: Microsatellite primers were developed to assess genetic diversity and population structure in Acer yangbiense , a critically endangered endemic species that occurs in northwestern Yunnan Province, China. • Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 34 microsatellite loci were isolated and characterized in A. yangbiense . Polymorphisms were evaluated in 39 individuals from two distinct populations, one of which was naturally occurring and the other an ex situ grouping. Nine of the markers showed polymorphisms with two to five alleles per locus; observed ( H o ) and expected ( H e ) heterozygosities ranged from 0.0000 to 0.8000 and from 0.0000 to 0.6889, respectively. • Conclusions: These microsatellite primers will prove useful in the further investigation of population genetics studies in A. yangbiense and, in addition, will assist related research of other congeneric species.