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Development of microsatellite loci for Cephalotaxus oliveri (Cephalotaxaceae) and cross‐amplification in Cephalotaxus
Author(s) -
Pan HuaWei,
Guo YingRong,
Su YingJuan,
Wang Ting
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100128
Subject(s) - microsatellite , biology , genetic diversity , locus (genetics) , amplified fragment length polymorphism , allele , genetics , evolutionary biology , gene , population , demography , sociology
• Premise of the study: Microsatellite loci were developed for Cephalotaxus oliveri , an endemic and endangered conifer in China, which will allow assessment of the levels of genetic diversity and a means to understand the genetic consequences of habitat fragmentation. • Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining (FIASCO) Repeats protocol, 19 microsatellite loci were identified in C. oliveri , 13 of which were polymorphic within a sample of 52 individuals representing five natural populations. The actual number of alleles per locus ranged from one to five. Twelve polymorphic loci were also successfully amplified in C. fortunei . • Conclusions: These microsatellite loci will provide a useful tool for further investigating genetic variation in natural populations of C. oliveri , which will inform future conservation and management strategies. Additionally, cross‐amplification in C. fortunei suggested the potential utility of these loci in this and other congeneric species.