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Development and characterization of polymorphic microsatellite primers in Reaumuria soongorica (Tamaricaceae)
Author(s) -
Chen Haikui,
Zhu Lili,
Cao Xiaohong,
Song Dandan,
Zhong Yang
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100112
Subject(s) - microsatellite , biology , locus (genetics) , genetic diversity , allele , genetics , population , evolutionary biology , genetic variation , gene , demography , sociology
• Premise of the study: Polymorphic microsatellite markers were developed in Reaumuria soongorica , a desert shrub occurring widely in western China, to investigate its genetic diversity and population genetic structure. • Methods and Results: Using the combined biotin capture technique, 10 novel polymorphic microsatellite loci primer sets were isolated and characterized in 90 individuals from six populations of R. soongorica . Five to 14 alleles per locus were identified for these microsatellites. Observed and expected heterozygosities ranged from 0.132 to 0.593, and 0.217 to 0.803, respectively. • Conclusions: These microsatellite markers provide a useful tool to assess the genetic variation and population structure of R. soongorica .