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Development and characterization of 20 novel polymorphic STS markers in Vicia faba (fava bean)
Author(s) -
Wang Haifei,
Yang Tao,
Guan Jianping,
Ma Yu,
Sun Xuelian,
Zong Xuxiao
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100092
Subject(s) - biology , vicia faba , germplasm , loss of heterozygosity , locus (genetics) , genetics , allele , microsatellite , primer (cosmetics) , population , genetic marker , botany , gene , chemistry , demography , organic chemistry , sociology
• Premise of the study: Sequence tagged site (STS) primers were developed for Vicia faba , based on amplified bands by ISSR primers. The usefulness of these STS markers was validated for size polymorphism among fava bean accessions. • Methods and Results: Based on the sequences derived from intersimple sequence repeat (ISSR) amplification, 66 sequence tagged site (STS) primer pairs were developed and screened, and 20 of them were polymorphic. The polymorphism of these markers was identified in 32 fava bean germplasm from different global geographical locations. Alleles ( N a ) per locus numbered 2 to 4, and expected heterozygosity ( H E ) per locus ranged from 0.000 to 0.714. There was significant variation in H E among germplasm from different regions for individual primers. • Conclusions: These novel polymorphic STS markers may be useful and convenient for further studies of population genetics, cultivar identification, and evolution in fava bean.