Development of microsatellite markers in Capsella rubella and Capsella bursa‐pastoris (Brassicaceae)

• Premise of the study: We developed microsatellite markers to investigate genetic diversity within and among populations of Capsella rubella and Capsella bursa‐pastoris and between these two species. • Methods and Results: Fourteen polymorphic microsatellite loci were identified in the two species and one more polymorphic microsatellite locus only in C. rubella . Samples from different European localities were genotyped. Up to six alleles per locus were observed in C. rubella , and up to 22 alleles per locus in C. bursa‐pastoris . Observed heterozygosities were low, indicating high selfing rates, especially in C. rubella . • Conclusions: The results provide valuable information on genetic diversity for future studies of population genetics in C. rubella and C. bursa‐pastoris .