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Isolation and characterization of tetranucleotide microsatellite loci in Pinus massoniana (Pinaceae)
Author(s) -
Guan Lanhua,
Shiraishi Susumu
Publication year - 2011
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1100076
Subject(s) - microsatellite , biology , pinus massoniana , pinaceae , locus (genetics) , population , dinucleotide repeat , transferability , primer (cosmetics) , genetics , allele , evolutionary biology , botany , pinus <genus> , gene , statistics , demography , mathematics , organic chemistry , logit , chemistry , sociology
• Premise of the study: Tetranucleotide microsatellite markers were developed for the first time in Pinus massoniana Lamb. to facilitate studies of population and conservation biology in this species. • Methods and Results: Ten tetranucleotide microsatellite primer pairs were developed using dual suppression PCR. Seven, six, and eight of the primer pairs exhibited cross‐species transferability to P. thunbergii , P. densiflora , and P. luchuensis , respectively. The number of alleles ranged from 1 to 31 per locus across four pine species. • Conclusions: Considering its advantage over dinucleotide microsatellites in generating fewer artifacts arising from stutter bands, this tetranucleotide microsatellite panel will facilitate future population and conservation biological studies in P. massoniana . Six to eight markers can also be used in studies of three congeneric species.